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BioTISR wide-field to 3D-SIM, live COS-7 cells

The wide-field fluorescence volume of one live COS-7 cell region at low excitation, its first time point, with the provider's higher-excitation 3D-SIM reconstruction as target: F-actin, microtubules or mitochondria.

10 test casescellsource wide-field, target 3D-SIM
Read before comparing (6)
  • Source. The provider's wide-field average at excitation level 1, linearly interpolated onto the SIM grid, which samples twice as finely in plane. It keeps its camera-count offset.
  • Target. The higher-excitation reconstruction, not a human annotation, normalised by its maximum with its negative values kept. The score's dynamic range is one.
  • Eligibility. prepare.py --survey finds a systematic wide-field/SIM displacement in 66 of 163 regions. Only the 97 regions displaced by under one SIM pixel are eligible; the corpus registers none of the others into eligibility.
  • Mask. The smoothed maximum projection of the target, thresholded, dilated by one micrometre and repeated through the slices. It is given to every method.
  • Spacing. In micrometres: 0.0306 in plane and 0.135 between slices on the target grid.
  • Donors. Region identifiers define the split; the release gives no higher-level donor split.

No method has a result on every case of this dataset yet.

AccuracyCost
#MethodMAEChangePSNR µmSSIMTime sRAM GBGPU GBImages
Source as given138.4 ±10.9-42.86 ±0.710.000 ±0.000

MAE: mean absolute error of a synthetic image against its target, inside the body, in the image's units. PSNR: the same by signal-to-noise ratio, higher is better. SSIM: the same by structural similarity, 1 is perfect.

Case by case

Click a score to look at that result: the target image, and over it the method's synthetic image. Underlined scores have their image; for the others you see the case without the result.

About the data

The first test case of BioTISR wide-field to 3D-SIM, live COS-7 cells
The first test caseClick to look at every case.
Test cases
Ten test regions and ten development regions drawn with seed 2026 by structure from the 97 eligible regions (prepare.py). Only each region's first time point is used; the later ones enter no split.
Truth
target image as released the provider 3D-SIM reconstruction of the same region and first time point, divided by its maximum value
Licence
CC BY 4.0
10 cases for tuning, never tested

actin_031, mt_009, mito_057, mito_042, actin_043, mt_020, mito_003, mito_058, actin_001, mt_012